LOCUS       40924_43238        6461 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pTha-K1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Tanaka R, Kikutani S, Mahardika A, Matsuda Y.
  TITLE     Localization of enzymes relating to C4 organic acid metabolisms in 
            the marine diatom, Thalassiosira pseudonana
  JOURNAL   Photosyn. Res. 121 (2-3), 251-263 (2014)
  PUBMED    24414292
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Kikutani S, Matsuda Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2013) Contact:Sae Kikutani Kwansei Gakuin 
            University, Department of Bioscience; 2-1 Gakuen, Sanda, Hyogo 
            669-1337, Japan
REFERENCE   3  (bases 1 to 6461)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6461)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Photosyn. 
            Res. 121 (2-3), 251-263 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-AUG-2013) Contact:Sae Kikutani Kwansei Gakuin University, 
            Department of Bioscience; 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      58..1023
                     /label=Tpfcp8
                     /note="Tpfcp8"
                     /regulatory_class="promoter"
     CDS             1024..1590
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     regulatory      1594..2096
                     /label=Tpfcp8
                     /note="Tpfcp8"
                     /regulatory_class="terminator"
     regulatory      2131..3145
                     /label=Tpfcp8
                     /note="Tpfcp8"
                     /regulatory_class="promoter"
     misc_feature    3146..3165
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      3166..3668
                     /label=Tpfcp8
                     /note="Tpfcp8"
                     /regulatory_class="terminator"
     primer_bind     complement(3700..3716)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(3858..4313)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4340..4444
                     /label=AmpR promoter
     CDS             4445..5302
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5476..6064
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6352..6373
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6388..6418
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6426..6442
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgaccatga ttacgccaag ctctaatacg actcactata gggaaagctt gcatgcctcc
       61 gagaactccc cataagtcaa cggctccaat caagaatgta tccgacaacg gcgagcatag
      121 caacacgtcc gtctttggag tagaatcatc atgttgtgga tgaatacaca gatgaatgac
      181 attaaaagca tgaacatgtt agagagtagg aggtagagat tgatatggta gcattgcgat
      241 gtttgttttt ggtcagcata tgatgagtgg ataccaatat gatgaaagtt gaatctcgcg
      301 tttgagctca gcggtacgtt attgatcgaa agtagcctga tcaaaatcct tggagagtac
      361 aagaggatca aagaatccag tgggggcgat aactccaagc tcgttctcaa agaggcaatg
      421 gaggtagaaa ctcatcccag ttgagaagaa gtgaaggcag tggcggtggc gaaagcagag
      481 gcaacgagga cagacttcct gtgggttgat gcaacgaata tttccagaag gagaagttta
      541 gagagttgaa ccgctaccta caatgacaaa gtatcgtatc gattttgatg ttggttggtt
      601 atgaattcaa actgtaagtt ggattgtgag aagatcagaa gttgaacgaa cacatctttc
      661 cgatcattca cctccacact gcaacaacac ggtacttctt ccgcggcagg tctctgtcgc
      721 cattctcttg tcctgttgtt ggctgtgaga cgaggaaagc aacgacaagt ttcacaaaag
      781 ggagttcctt taacgagata tgttttttat aaagagtccc aatagaaaga caaattgatt
      841 cctccgtgca aacgcgcaaa taaacaccac gtccattata tccatatctt tcagagtatc
      901 caacaagtgt tgaaggacag gtagttgaag taacgtatct tccccctcga ctggatccat
      961 caacaaggcg aacaaatcca ttcaacctct cataaattat ctgatttacc aaaccgatac
     1021 caaatgacca ctcttgacga cacggcttac cggtaccgca ccagtgtccc gggggacgcc
     1081 gaggccatcg aggcactgga tgggtccttc accaccgaca ccgtcttccg cgtcaccgcc
     1141 accggggacg gcttcaccct gcgggaggtg ccggtggacc cgcccctgac caaggtgttc
     1201 cccgacgacg aatcggacga cgaatcggac gccggggagg acggcgaccc ggactcccgg
     1261 acgttcgtcg cgtacgggga cgacggcgac ctggcgggct tcgtggtcgt ctcgtactcc
     1321 ggctggaacc gccggctgac cgtcgaggac atcgaggtcg ccccggagca ccgggggcac
     1381 ggggtcgggc gcgcgttgat ggggctcgcg acggagttcg cccgcgagcg gggcgccggg
     1441 cacctctggc tggaggtcac caacgtcaac gcaccggcga tccacgcgta ccggcggatg
     1501 gggttcaccc tctgcggcct ggacaccgcc ctgtacgacg gcaccgcctc ggacggcgag
     1561 caggcgctct acatgagcat gccctgcccc tgaatactgg attggtgaat caatgagccg
     1621 tagcacaatg gttacattcg gctagctaag atccaatggc aaggaccaag tgctggaact
     1681 tgttttgctt tagcagatct tagcgtgaga ggtatttgtc ctctgtcagg agtagatagt
     1741 agatgttctt tttaaactaa aatgctaact gttccgaatt cctcatcgca gctaatccgt
     1801 acatcaaaag acaaaatgct aggtatgtgt actacatctc ctgttgctag ataagacata
     1861 tgataggaaa cacaccatca atagtcattg tagctttact tatactacgc atttgcactt
     1921 tcccctgagt ggcagaggcg cattgagaaa atcgatctca acatagttta tgtagcatcc
     1981 cctagatcca ttacgttaag tctccttcgt ctttggtgta ggcatgttgg acacaacgag
     2041 gtaaaacaca acacaaacaa tgtgtccagc aaagtagtag ctgctccagt tctcccggtc
     2101 gactctagag gatctactag tcatatggat caaggacggc atccatggca aaacgtccga
     2161 gatttagctt ctgaagcttg cgctttttcc gagaactccc cataagtcaa cggctccaat
     2221 caagaatgta tccgacaacg gcgagcatag caacacgtcc gtctttggag tagaatcatc
     2281 atgttgtgga tgaatacaca gatgaatgac attaaaagca tgaacatgtt agagagtagg
     2341 aggtagagat tgatatggta gcattgcgat gtttgttttt ggtcagcata tgatgagtgg
     2401 ataccaatat gatgaaagtt gaatctcgcg tttgagctca gcggtacgtt attgatcgaa
     2461 agtagcctga tcaaaatcct tggagagtac aagaggatca aagaatccag tgggggcgat
     2521 aactccaagc tcgttctcaa agaggcaatg gaggtagaaa ctcatcccag ttgagaagaa
     2581 gtgaaggcag tggcggtggc gaaagcagag gcaacgagga cagacttcct gtgggttgat
     2641 gcaacgaata tttccagaag gagaagttta gagagttgaa ccgctaccta caatgacaaa
     2701 gtatcgtatc gattttgatg ttggttggtt atgaattcaa actgtaagtt ggattgtgag
     2761 aagatcagaa gttgaacgaa cacatctttc cgatcattca cctccacact gcaacaacac
     2821 ggtacttctt ccgcggcagg tctctgtcgc cattctcttg tcctgttgtt ggctgtgaga
     2881 cgaggaaagc aacgacaagt ttcacaaaag ggagttcctt taacgagata tgttttttat
     2941 aaagagtccc aatagaaaga caaattgatt cctccgtgca aacgcgcaaa taaacaccac
     3001 gtccattata tccatatctt tcagagtatc caacaagtgt tgaaggacag gtagttgaag
     3061 taacgtatct tccccctcga ctggatccat caacaaggcg aacaaatcca ttcaacctct
     3121 cataaattat ctgatttacc aaaccgatat cgcatgcgcg gccgcatact ggattggtga
     3181 atcaatgagc cgtagcacaa tggttacatt cggctagcta agatccaatg gcaaggacca
     3241 agtgctggaa cttgttttgc tttagcagat cttagcgtga gaggtatttg tcctctgtca
     3301 ggagtagata gtagatgttc tttttaaact aaaatgctaa ctgttccgaa ttcctcatcg
     3361 cagctaatcc gtacatcaaa agacaaaatg ctaggtatgt gtactacatc tcctgttgct
     3421 agataagaca tatgatagga aacacaccat caatagtcat tgtagcttta cttatactac
     3481 gcatttgcac tttcccctga gtggcagagg cgcattgaga aaatcgatct caacatagtt
     3541 tatgtagcat cccctagatc cattacgtta agtctccttc gtctttggtg taggcatgtt
     3601 ggacacaacg aggtaaaaca caacacaaac aatgtgtcca gcaaagtagt agctgctcca
     3661 gttctcccaa tcggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg
     3721 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     3781 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     3841 cctgaatggc gaatgggaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
     3901 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3961 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4021 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4081 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4141 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4201 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4261 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     4321 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4381 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4441 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4501 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4561 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4621 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4681 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4741 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4801 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4861 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4921 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4981 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5041 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5101 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5161 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5221 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5281 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5341 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5401 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5461 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5521 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5581 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5641 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5701 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5761 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5821 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5881 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5941 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6001 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6061 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6121 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6181 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6241 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6301 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     6361 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     6421 tggaattgtg agcggataac aatttcacac aggaaacagc t
//
